The Canada Border Services Agency says the Ambassador Bridge border crossing between Windsor, Ont., and Detroit has reopened.
The crossing -- one of the busiest in the country -- had been closed for nearly a week by a blockade protesting COVID-19 measures.
The bridge sees hundreds of millions of dollars in imports and exports cross it each day, and politicians on both sides of the border decried the economic impact of the protest.
Police removed protesters from the site on Sunday, making more than two dozen arrests.
They moved in after the Ontario Superior Court granted an injunction Friday barring protesters from blocking the bridge.
Windsor Mayor Drew Dilkens characterized the protest as an ``illegal occupation'' of the bridge.